Composite

Part:BBa_K4940011:Design

Designed by: Kuang Minjie   Group: iGEM23_iZJU-China   (2023-10-11)


Transcription unit of P450BM-3 QM-his and GlcDH-II-his


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1441
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1441
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1441
    Illegal XhoI site found at 1952
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1441
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1441
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1287


Design Notes

The main idea was to have a high expression of the gene and the expression should be controlled safely. In that case, commercial T7 promoter is useful and lac operator represses the gene expression without IPTG induction.

Source

Most of the parts are already in the commercial plasmids, and we select a proper vector to use. The gene sequence was found in NCBI and synthesized by the company.

References